Thermo Fisher Scientific Leptin Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC.

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Rat Leptin. The anti-Rat Leptin-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Rat Leptin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat Leptin (500-P185G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Rat Leptin.

Western Blot:
To detect Rat Leptin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant Rat Leptin is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P185GBT-1MG will be provided as 2 × 500 µg.

Target Information

Leptin is a 167 amino acid-long protein hormone (~16 kDa) that regulates body weight, metabolism, and reproductive function. It is encoded by the obese (ob) gene and primarily expressed by adipocytes. Leptin receptors are highly expressed in the hypothalamus, T lymphocytes, and vascular endothelial cells. Leptin influences feeding behavior, energy expenditure, body temperature regulation, hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, pubertal development, and fetal growth. It has been implicated in obesity, anorexia nervosa, insulin resistance, and hypertension. Defects in leptin production can cause severe hereditary obesity in rodents and humans.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.