Thermo Fisher Scientific CD178 (soluble) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL.

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human sFas Ligand. Anti-Human sFas Ligand-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hFasL/Apo1L by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human FasL/Apo1L (500-P184G) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hFasL/Apo1L.

Western Blot:
To detect Human sFas Ligand by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 mg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human sFas Ligand is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package:
500-P184GBT-1MG will be provided as 2 × 500 µg.

Target Information

CD178 (Fas ligand, FasL) is a type-II membrane protein with its N-terminus in the cytoplasm and C-terminal region extending extracellularly. Its receptor, FasR, is a type-I membrane protein and a member of the TNF and NGF receptor family. As a TNF-cytokine family member, CD178 induces apoptosis upon binding to FasR.

CD178 exists in membrane-bound (45 kD) and soluble (26 kD) forms, the latter released by metalloproteinase cleavage. Binding of CD178 to Fas triggers receptor oligomerization and apoptotic signaling. CD178 is expressed mainly in activated T-lymphocytes, NK cells, and immune-privileged tissues such as testis and eye. Expression has also been reported in thymus, liver, ovary, lung, heart, and kidney.

The Fas/CD178 system is involved in immune regulation, T cell-mediated cytotoxicity, and pathogenesis of diseases such as AIDS, hepatitis, cancer, atherosclerosis, and inflammatory disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.