Thermo Fisher Scientific ERBB3 Monoclonal Antibody (OTI3C1), TrueMAB
ERBB3 단백질을 표적하는 Mouse IgG1 단클론 항체로, WB와 IHC(P)에서 검증됨. Human, Mouse, Rat 반응성. Lyophilized 형태로 제공되며, PBS/trehalose buffer에 보관. 연구용으로 세포 신호전달 및 종양 연구에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3C1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 983–1342 of human ERBB3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ErbB3 (c-erbB-3/HER-3) is a member of the type I family of growth factor receptors that binds to ligands in the heregulin family.
It is over-expressed in various tumors including prostate, bladder, breast, stomach, pancreas, and colon.
Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents.
The ErbB3 gene is located on chromosome 12q13 and encodes a 160/180 kDa transmembrane glycoprotein receptor tyrosine kinase.
ErbB3 forms heterodimers with other EGF receptor family members, activating pathways leading to cell proliferation or differentiation.
Alternate splice variants include a secreted isoform lacking the transmembrane region, which modulates the activity of the membrane-bound form.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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