Thermo Fisher Scientific HLA-Pan (MHC II) Recombinant Rabbit Monoclonal Antibody (HLA-Pan, 2967R)
HLA class II 분자 검출용 재조합 토끼 단클론 항체. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. 인간 시료 반응성, Protein A/G 정제, 액상 형태로 제공. 면역펩티도믹스 연구 및 항원 제시 세포 분석에 적합.
- 카탈로그번호
- RBM1-2967-P0
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HLA-Pan, 2967R |
| Immunogen | Non-T, non-B human acute lymphoblastic leukemia REH6 cell line. Targets include HLA-DR/DP/DQ family (UniProt IDs: P01911-1, P01920-1, P04440-1). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
MHC class II molecules are transmembrane glycoproteins composed of an alpha chain (36 kDa) and a beta chain (27 kDa). They are primarily expressed on antigen-presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes.
Three loci—DR, DQ, and DP—encode the major expressed products of the human class II region. These molecules bind intracellularly processed peptides and present them to T-helper cells, playing a critical role in initiating the immune response.
HLA and MHC antibodies are essential tools in immunopeptidomics, aiding in the identification and characterization of neoantigens through high-performance liquid chromatography coupled with tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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