Thermo Fisher Scientific Id3 Polyclonal Antibody
Id3 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, Flow cytometry에 적합합니다. Human 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Flow Cytometry (Flow) | 0.5–5 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide of human ID3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human colon
- Cellular location: Nucleus
Target Information
Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3, and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The ID-type proteins thus appear to negatively regulate DNA binding of bHLH proteins.
Since ID1 inhibits DNA binding of E12 and Myo D, it functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, ID protein levels decrease, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which activate myogenic differentiation. Expression of each ID protein is strongly dependent on growth factor activation, and reduction of ID mRNA levels by antisense oligonucleotides leads to delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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