Thermo Fisher Scientific S100A9 + Calprotectin (S100A8/A9 Complex) Recombinant Rabbit Monoclonal Antibody (MAC3157R)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

-

Product Specifications

Species Reactivity

Baboon, Bovine, Dog, Horse, Cat, Guinea pig, Goat, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

MAC3157R

Immunogen

Affinity Purified monocyte membrane preparation if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calprotectin,uniProtId:P06702-1,ncbiNodeId:9606,antigenRange:1-114,antigenLength:114,antigenImageFileName:6279-RBM5-P1_Calprotectin_P06702-1_Rabbit.svg,antigenImageFileNamePDP:6279-RBM5-P1_Calprotectin_P06702-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Calprotectin,uniProtId:P05109-1,ncbiNodeId:9606,antigenRange:1-93,antigenLength:93,antigenImageFileName:6279-RBM5-P1_Calprotectin_P05109-1_Rabbit.svg,antigenImageFileNamePDP:6279-RBM5-P1_Calprotectin_P05109-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.