Thermo Fisher Scientific S100A9 + Calprotectin (S100A8/A9 Complex) Recombinant Rabbit Monoclonal Antibody (MAC3157R)
S100A9 및 Calprotectin(S100A8/A9 복합체)을 인식하는 재조합 토끼 단클론 항체. 다양한 종에 반응하며 IHC(P)에서 1–2 µg/mL로 사용 가능. 고순도 Protein A/G 정제, 보존제 무첨가 PBS 용액. 염증 관련 연구에 적합.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Baboon, Bovine, Dog, Horse, Cat, Guinea pig, Goat, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | MAC3157R |
| Immunogen | Affinity purified monocyte membrane preparation |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For Immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
The S100A8/A9 heterocomplex, also known as MRP-8/MRP-14 complex or calprotectin, constitutes approximately 60% of the cytoplasmic protein fraction in circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages, and ileal tissue eosinophils.
Peripheral blood monocytes carry the antigen both extra- and intracellularly, while neutrophils contain it only intracellularly.
The S100A8/A9 complex exhibits antibacterial, antifungal, immunomodulatory, and antiproliferative properties and acts as a potent chemotactic factor for neutrophils.
Plasma concentrations increase in conditions associated with elevated neutrophil activity, such as inflammatory bowel disease.
Calprotectin is detectable in feces due to granulocyte transmigration through the intestinal wall.
Elevated calprotectin levels have been observed in plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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