Thermo Fisher Scientific GFP Polyclonal Antibody
GFP 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. 높은 특이성과 감도를 제공하며, Aequorea victoria 유래 GFP 전장 단백질로부터 제작. 연구용으로만 사용 가능.
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- 600-401-215x (3개 옵션)
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카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific GFP Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:200–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:5,000 |
| ELISA | 1:20,000–1:120,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Green Fluorescent Protein (GFP) fusion protein corresponding to the full-length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.99 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid repeated freeze–thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay Results:
Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified/partially purified GFP (Aequorea victoria).
No cross-reactivity observed with Human, Mouse, or Rat serum proteins.
Target Information
The jellyfish Aequorea victoria produces Green Fluorescent Protein (GFP), which emits green light during bioluminescence. GFP is widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic systems, and as a fusion tag for protein localization and movement studies.
- GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autocatalytically.
- Absorbs blue light (max 395 nm) and emits green light (peak 508 nm).
- Functions independently of additional proteins, substrates, or cofactors.
- GFP fluorescence is stable and species-independent.
- Commonly used in gene expression monitoring, protein localization, protein–protein interaction assays, and FRET experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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