Thermo Fisher Scientific TPH1 Recombinant Rabbit Monoclonal Antibody (SC53-07)
TPH1 단백질을 인식하는 재조합 토끼 모노클로날 항체로 높은 특이성과 민감도를 제공합니다. WB, IHC, ICC/IF, Flow Cytometry에 적합하며, 일관된 로트 간 품질을 유지합니다. Protein A로 정제된 액상 형태로 장기 보관 시 -20°C에서 안정적입니다.
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Thermo Fisher Scientific TPH1 Recombinant Rabbit Monoclonal Antibody (SC53-07)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC53-07 |
| Immunogen | Synthetic peptide within Human TPH1 (aa 372–419) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809496 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, initiating the biosynthesis of serotonin and melatonin.
In mammals:
- Serotonin is synthesized mainly in neurons from the Raphe nuclei.
- Melatonin synthesis occurs in the pineal gland.
TPH activity is rate-limiting for serotonin but not melatonin biosynthesis.
The enzyme’s activity is enhanced by phosphorylation via PKA and Ca²⁺/calmodulin kinase II (CAM K II), both acting on Ser58 within the regulatory domain of TPH.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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