Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), NovaFluor Red 755, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.2 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin, eFluor 450, FITC
• Functional Grade
• NovaFluor Blue/Red/Violet/Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright Series
• Custom conjugation available

Product Specific Information

The OKT8 monoclonal antibody reacts with the human CD8a molecule (32–34 kDa), expressed as a heterodimer (CD8αβ) or homodimer (CD8αα). CD8a is found on most thymocytes and subsets of mature T cells and NK cells. It binds to MHC class I and associates with tyrosine kinase p56lck, playing a key role in T-cell development and activation.

Preliminary testing indicates OKT8, RPA-T8, and HIT8a clones do not compete for binding to human leukocytes, suggesting distinct epitopes.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for human peripheral blood cells. Use 5 µL (0.2 µg)/test in 100 µL final volume. Suitable for 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Product # C001T06F01) must be used for optimal staining. Add 5 µL per sample or per 100 µL of cell suspension for single-color controls.
• NovaFluor conjugates utilize Phiton™ technology for engineered fluorescent signatures minimizing spillover and spread.

Excitation: 636 nm
Emission: 756 nm
Laser: 633–640 nm (Red)

Target Information

CD8 is a type I transmembrane glycoprotein of the immunoglobulin family, functioning as a co-receptor with TCR for MHC class I molecules. It is primarily expressed on cytotoxic T cells, NK cells, monocytes, and dendritic cells, existing as CD8αβ heterodimers or CD8αα homodimers. Binding of CD8 to MHC-I/peptide complexes triggers Lck activation, cytokine production, and cytotoxic T lymphocyte (CTL) activation. Activated CTLs are essential for pathogen and tumor cell clearance. Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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