Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), NovaFluor Red 710, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.1 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin, eFluor 450, FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright Series
• Custom conjugation available

Product Specific Information

The OKT8 monoclonal antibody reacts with human CD8a, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimers or CD8αα homodimers. CD8a is found on most thymocytes and subsets of mature T cells and NK cells. It binds MHC class I molecules and, through association with tyrosine kinase p56lck, participates in T-cell development and activation.

Preliminary testing shows that OKT8 does not compete with clones RPA-T8 or HIT8a for binding to human peripheral blood leukocytes, indicating distinct epitopes.

Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Applications Reported

• Flow cytometric analysis

Applications Tested

• Pre-titrated and tested for flow cytometry on normal human peripheral blood cells
• Recommended use: 5 µL (0.1 µg)/test in 100 µL final volume
• Suitable for 10⁵–10⁸ cells/test

Handling Notes

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• Use CellBlox Plus Blocking Buffer (Product # C001T06F01) for optimal staining.
• Add 5 µL of CellBlox Plus per sample prior to combining with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).
• NovaFluor conjugates utilize Phiton™ technology for engineered fluorescent signatures with minimized spillover and spread.

Excitation: 639 nm
Emission: 710 nm
Laser: 633–640 nm (Red)

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family. It functions as a co-receptor with TCR for MHC class I molecules and plays a key role in T-cell activation and differentiation. CD8 is mainly expressed on cytotoxic T cells, NK cells, monocytes, and dendritic cells. Upon MHC-I/peptide complex ligation, it recruits Lck kinase, triggering signaling cascades leading to CTL activation and pathogen or tumor cell clearance. Differentiation of naïve CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.