Thermo Fisher Scientific CXCL1 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human GRO-alpha/MGSA (CXCL1) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CXCL1 (GRO alpha),uniProtId:P09341-1,ncbiNodeId:9606,antigenRange:35-107,antigenLength:107,antigenImageFileName:500-P92BT-500UG_CXCL1_GRO_alpha_P09341-1_Rabbit.svg,antigenImageFileNamePDP:500-P92BT-500UG_CXCL1_GRO_alpha_P09341-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

AA Sequence of recombinant protein: ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-alpha/MGSA (CXCL1). Anti-Human GRO-alpha/MGSA (CXCL1)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human GRO-alpha/MGSA by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GRO-alpha/MGSA (500-P92) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GRO-alpha/MGSA.

Western Blot: To detect Human GRO-alpha/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human GRO-alpha/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P92BT-1MG will be provided as 2 x 500 µg

Target Information

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.