Thermo Fisher Scientific Phospho-GATA3 (Ser162) Polyclonal Antibody
GATA3 단백질의 Ser162 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. Human, Mouse, Rat에 반응. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human GATA3 around the phosphorylation site of Ser162 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The genes for all four subunits of the T-cell antigen receptor (alpha, beta, gamma, and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all four TCR genes. GATA3, an enhancer-binding protein with a zinc finger domain, is expressed predominantly in T cells and is believed to regulate T-cell receptor gene activation through enhancer binding. The human GATA3 gene comprises six exons distributed over 17 kb of DNA, containing two zinc fingers encoded by separate exons conserved with GATA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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