Thermo Fisher Scientific PDPK1 Polyclonal Antibody
Thermo Fisher Scientific의 PDPK1 Polyclonal Antibody는 인간, 마우스, 비인간 영장류, 랫트에 반응합니다. Western Blot 및 IHC(PFA fixed)에 적합하며, 액상 형태로 1 mg/mL 농도입니다. PDPK1 단백질의 내인성 수준 검출에 사용되며, 대사 연구용으로 이상적입니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (PFA fixed) (IHC (PFA))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PDPK1 (Accession O15530), corresponding to amino acid residues L350–S400 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2900368 |
Product Specific Information
Antibody detects endogenous levels of total PDPK1.
Target Information
PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. It also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), leading to activation of glycogen synthase and stimulation of glycogen synthesis.
Because of its role in insulin-induced glycogen synthesis and PKC activation, PDPK1 is a potentially important target for metabolic drug research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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