Thermo Fisher Scientific Phospho-RSK Pan (Ser221, Ser227, Ser218, Ser232) Polyclonal Antibody
RSK1~4의 인산화 부위(Ser221, Ser227, Ser218, Ser232)를 인식하는 Rabbit Polyclonal Antibody. Western blot에 최적화되어 있으며 HepG2 세포를 양성 대조군으로 권장. ERK 경로의 말단 키나아제 RSK 활성 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
- Suggested Positive Control: HepG2 cells
- View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of Serine 221/227/218/232 (A-Y-S(p)-F-C) derived from Human RSK1/2/3/4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554437 |
Target Information
The p90 ribosomal S6 kinases (RSKs) are a family of serine/threonine kinases at the terminus of the ERK pathway. In humans, four isoforms (RSK1–4) exist. Each RSK contains two functional kinase domains activated sequentially by phosphorylation.
RSKs regulate gene expression by phosphorylating transcriptional regulators such as c-Fos, estrogen receptor, NFκB/IκBα, and CREB. ERK activates the C-terminal kinase, which then activates the N-terminal kinase.
RSK proteins localize to both cytoplasm and nucleus. Upon growth factor stimulation, cytosolic RSK translocates to the nucleus, leading to phosphorylation and activation of transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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