Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Tyr204) Polyclonal Antibody
ERK1/ERK2 인산화(Thr202, Tyr204) 부위를 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF 등에 사용 가능. Human, Mouse, Rat 반응성. 액상 형태, 1 mg/mL 농도, -20°C 보관. 세포 신호전달 연구에 적합.
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Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Tyr204) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:200 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of Threonine 202/Tyrosine 204 (F-L-T(p)-E-Y(p)-V-A) derived from Human p44/42 MAP Kinase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554436 |
Product Specific Information
A suggested positive control for Western blot is NIH-3T3 cells.
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
Various stimuli such as growth factors, cytokines, virus infection, and transforming agents activate the ERK1/ERK2 pathways.
Upon activation, ERKs phosphorylate cytoplasmic targets or migrate to the nucleus to activate transcription factors including c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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