Thermo Fisher Scientific KDEL Monoclonal Antibody (1F2), FITC
KDEL 단백질 검출용 FITC 결합 마우스 모노클론 항체로, ER 유지 단백질 연구에 적합. Western blot에서 1:1,000 희석으로 사용 가능. 498/517 nm 형광 특성, 액상 형태로 4°C 암소 보관. 연구용 전용 제품.
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Applications
- Western Blot (WB)
Tested Dilution: 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1F2 |
| Immunogen | KDEL peptide (C-AVKDEL-COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45866 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects KDEL-tagged proteins.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins.
GRP78 (78 kDa) and GRP94 (94 kDa) glucose-regulated proteins, as well as protein disulfide isomerase (PDI), share the C-terminal KDEL sequence.
The presence of carboxy-terminal KDEL is necessary for ER retention and sufficient to reduce secretion of proteins from the ER. This retention is mediated by a KDEL receptor.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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