Thermo Fisher Scientific 4-Hydroxy-2-hexenal Monoclonal Antibody (5C11.1), PE
4-Hydroxy-2-hexenal을 검출하기 위한 PE-conjugated mouse monoclonal antibody. Western blot, ELISA, Dot blot에 사용 가능. 높은 특이성과 낮은 교차반응성을 보이며, 565/576 nm 형광 특성. 연구용으로만 사용.
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Thermo Fisher Scientific 4-Hydroxy-2-hexenal Monoclonal Antibody (5C11.1), PE
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:1,000 |
| Dot Blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5C11.1 |
| Immunogen | Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932314 |
Available Formats
- Unconjugated (MA5-27576)
- APC (MA5-45857)
- FITC (MA5-45858)
- PerCP (MA5-45859)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45860 was sufficient for detection of 4-Hydroxy-2-hexenal in 1 µg of 4-Hydroxy-2-hexenal-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
4-Hydroxynonenal (4-HNE, C9H16O2) is an alpha,beta-unsaturated hydroxyalkenal produced by lipid peroxidation in cells. It is the primary alpha,beta-unsaturated hydroxyalkenal formed during this process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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