Thermo Fisher Scientific 4-Hydroxy-2-hexenal Monoclonal Antibody (12F4), PE
4-Hydroxy-2-hexenal을 특이적으로 검출하는 Mouse IgG1 단클론 항체. PE 형광 표지로 565/576 nm에서 Excitation/Emission. Western blot, ELISA, Dot blot에 1:1,000 희석으로 사용 가능. 연구용으로만 사용 가능.
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Thermo Fisher Scientific 4-Hydroxy-2-hexenal Monoclonal Antibody (12F4), PE
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:1,000 |
| Dot Blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12F4 |
| Immunogen | Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation/Emission Max | 565/576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45864 was sufficient for detection of 4-Hydroxy-2-hexenal in 1 µg of 4-Hydroxy-2-hexenal-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
4-Hydroxynonenal (4-HNE, C9H16O2) is an alpha,beta-unsaturated hydroxyalkenal produced by lipid peroxidation in cells. 4-HNE is the primary alpha,beta-unsaturated hydroxyalkenal formed in this process.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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