Thermo Fisher Scientific Biotin (Vitamin B7 or Vitamin H) Monoclonal Antibody (BTN, 403)
Biotin(Vitamin B7/H)에 특이적인 Mouse Monoclonal Antibody로 다양한 면역실험(WB, IHC, ICC, Flow 등)에 사용 가능. Protein A/G로 정제된 액상 형태이며, 4°C 보관. 연구용으로만 사용.
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Thermo Fisher Scientific Biotin (Vitamin B7 or Vitamin H) Monoclonal Antibody (BTN, 403)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
| Immunomicroscopy (IM) | Assay-dependent |
| In Situ Hybridization (ISH) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BTN, 403 |
| Immunogen | Biotinylated sheep immunoglobulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer (pH 6.0) for 10–20 minutes, followed by cooling at room temperature for 20 minutes.
Target Information
Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is essential for the incorporation of carbon dioxide into organic compounds and exhibits a molecular weight of approximately 244 Da.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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