Thermo Fisher Scientific Biotin (Vitamin B7 or Vitamin H) Monoclonal Antibody (BTN, 403)
Biotin(Vitamin B7) 인식 단일클론 항체로 Western Blot, IHC, ICC, Flow Cytometry 등 다양한 응용 가능. Mouse IgG1(kappa) 형식, 비결합 상태 액상 제품. 고순도 Protein A/G 정제, 안정적 PBS buffer 저장. 연구용 전용 시약.
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Thermo Fisher Scientific Biotin (Vitamin B7 or Vitamin H) Monoclonal Antibody (BTN, 403)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
| Immunomicroscopy (IM) | Assay-dependent |
| In Situ Hybridization (ISH) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BTN, 403 |
| Immunogen | Biotinylated sheep immunoglobulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature (RT).
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer (pH 6.0) for 10–20 minutes, followed by cooling at RT for 20 minutes.
Target Information
Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7.
It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species.
Biotin is essential for incorporation of carbon dioxide into organic compounds and has a molecular weight of approximately 244 Da.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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