Thermo Fisher Scientific TRAIL Polyclonal Antibody, PeproTech
마우스 TRAIL 단백질을 인식하는 Rabbit Polyclonal 항체로, Western Blot과 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 리오필라이즈드 형태로 제공됩니다. 세포사멸 연구 및 TRAIL 관련 신호전달 연구에 활용 가능합니다.
- 카탈로그번호
- 500-P303-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TRAIL Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine TRAIL |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930307 |
Product Specific Information
AA Sequence of recombinant protein:
MRGGRPQKVA AHITGITRRS NSALIPISKD GKTLGQKIES WESSRKGHSF LNHVLFRNGE LVIEQEGLYY IYSQTYFRFQ EAEDASKMVS KDKVRTKQLV QYIYKYTSYP DPIVLMKSAR NSCWSRDAEY GLYSIYQGGL FELKKNDRIF VSVTNEHLMD LDQEASFFGA FLIN.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine TRAIL. Anti-Murine TRAIL-specific antibody was purified by affinity chromatography employing an immobilized Murine TRAIL matrix.
Sandwich ELISA:
To detect Murine TRAIL by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen-affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine TRAIL (500-P303Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine TRAIL.
Western Blot:
To detect Murine TRAIL by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Murine TRAIL is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
TRAIL is a cytokine belonging to the tumor necrosis factor (TNF) ligand family. It preferentially induces apoptosis in transformed and tumor cells but does not appear to kill normal cells, although expressed in most normal tissues.
TRAIL binds to several members of the TNF receptor superfamily, including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly TNFRSF11B/OPG.
Its activity may be modulated by decoy receptors (TRAILR3, TRAILR4, OPG) that cannot induce apoptosis. Binding of TRAIL to its receptors triggers activation of MAPK8/JNK, caspase 8, and caspase 3.
Receptors for TRAIL include two death domain-containing receptors (DR4 and DR5) and two decoy receptors (DcR1 and DcR2). TRAIL is a type II membrane protein expressed in various human tissues and induces apoptosis and NF-κB activation similar to TNF and Fas ligand.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.