Thermo Fisher Scientific GTF2E1/TFIIE-alpha Polyclonal Antibody
인간 GTF2E1/TFIIE-alpha를 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, IP에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 1 mg/mL 농도. FFPE 조직에서는 citrate buffer pH 6.0으로 epitope retrieval 권장.
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Thermo Fisher Scientific GTF2E1/TFIIE-alpha Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 100 and 150 of human General Transcription Factor IIE, polypeptide 1, alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, predicted to react with Bovine and Orangutan
Target Information
The general transcription factor TFIIE plays an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general transcription factors.
Human TFIIE consists of two subunits: TFIIE-alpha (57 kDa) and TFIIE-beta (34 kDa), joining the preinitiation complex after RNA polymerase II and TFIIF.
TFIIE-alpha is necessary for transcription initiation and can fully replace the natural subunit in vitro.
Its sequence includes structural motifs such as leucine repeat, zinc finger, and helix-turn-helix, showing similarity to bacterial sigma factors involved in transcription regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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