Thermo Fisher Scientific GTF2E1/TFIIE-alpha Polyclonal Antibody
Rabbit polyclonal antibody against human GTF2E1/TFIIE-alpha. Validated for WB, IHC, IHC-P, and IP. Recommended dilutions: 1:2,000–1:10,000 (WB), 1:100–1:500 (IHC). Liquid form, 0.20 mg/mL, stored at 4°C. Predicted cross-reactivity with bovine and orang...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 389 and 439 of human General Transcription Factor IIE, polypeptide 1, alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with bovine and orangutan
Target Information
The general transcription factor TFIIE plays an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors.
Human TFIIE consists of two subunits of relative molecular mass 57,000 (TFIIE-alpha) and 34,000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF.
TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay.
Its sequence contains several structural motifs (leucine repeat, zinc finger, helix-turn-helix) and similarities to bacterial sigma factors, suggesting a direct involvement in transcription regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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