Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), eBioscience

Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• APC
• Biotin
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Yellow 570
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation available

Product Specific Information

Description:
The AER-37 monoclonal antibody reacts with the FcεRIα subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIα is expressed on mast and basophil cells and is upregulated by the presence of IgE. It forms a tetrameric complex with one beta and two gamma subunits, which possess ITAM motifs. The FcεRI complex plays a key role in triggering IgE-mediated allergic reactions.

Applications Reported:
AER-37 (CRA1) antibody is reported for use in flow cytometric analysis and does not block IgE binding.

Applications Tested:
Tested by flow cytometric analysis of peripheral blood cells at ≤1 µg/test. A test is defined as the amount (µg) of antibody staining a cell sample in 100 µL. Recommended to titrate for optimal performance.
Cell number range: 10⁵–10⁸ cells/test.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

FceR1 (Fc epsilonR1 alpha) is a subunit of the high-affinity receptor for IgE. It forms a tetrameric complex (one alpha, one beta, two gamma subunits). The receptor initiates allergic responses by coupling allergen-bound IgE with mast cells, leading to mediator release such as histamine, prostaglandins, and leukotrienes—key effectors of hypersensitivity reactions like hay fever and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.