Thermo Fisher Scientific CD254 (RANK Ligand) Monoclonal Antibody (IK22/5), eBioscience
Mouse CD254(RANKL/TRANCE) 단백질을 인식하는 Rat IgG2a 단클론 항체로, Flow Cytometry 등 다양한 응용 가능. 0.5 mg/mL 액상 형태, 4°C 보관. Osteoclast 분화 및 면역세포 상호작용 연구에 적합.
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- 14-5952-8x (2개 옵션)
- 판매단위
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Thermo Fisher Scientific CD254 (RANK Ligand) Monoclonal Antibody (IK22/5), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 2 publications |
| Functional Assay (Functional) | Assay-Dependent | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IK22/5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467745 |
Additional Formats Available:
- Biotin ([13-5952-82])
- Functional Grade ([16-5952-85])
- PE ([12-5952-82])
- Custom Conjugation Request Available
Product Specific Information
The IK22/5 monoclonal antibody reacts with mouse CD254, also known as TRANCE (TNF-related activation-induced cytokine receptor), RANKL (receptor activator of NF-kappa B ligand), and OPGL (Osteoprotegerin Ligand).
TRANCE is a type II membrane protein (~35 kDa) closely related to TRAIL, FasL, and TNF. It is upregulated by TCR stimulation and expressed abundantly by mouse T cells in thymus and lymph nodes.
TRANCE-deficient mice lack osteoclasts and show defects in T-dendritic and T-B cell interactions, critical for osteoclast differentiation. The TRANCE-RANK interaction regulates expression of the anti-apoptotic molecule Bcl-xL.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Validated on mouse CD254/TRANCE-transfected cells. Recommended ≤0.5 µg/test in 100 µL final volume (10^5–10^8 cells/test). Antibody titration is advised for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD254 (RANKL/TRANCE) is a TNF family cytokine ligand for osteoprotegerin, essential for osteoclast differentiation and activation. It acts as a dendritic cell survival factor and regulates T cell-dependent immune responses.
Activation of T cells induces CD254 expression, promoting osteoclastogenesis and bone loss. It activates anti-apoptotic kinase AKT/PKB via SRC kinase and TRAF6 complex, implicating a role in apoptosis regulation.
Gene disruption in mice causes severe osteopetrosis, lack of osteoclasts, and defects in T/B lymphocyte differentiation and mammary gland development. Two alternatively spliced transcript variants are known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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