Thermo Fisher Scientific CD254 (RANK Ligand) Monoclonal Antibody (IK22/5), eBioscience

Thermo Fisher Scientific CD254 (RANK Ligand) Monoclonal Antibody (IK22/5), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available:

• Biotin ([13-5952-82])
• Functional Grade ([16-5952-85])
• PE ([12-5952-82])
• Custom Conjugation Request Available

Product Specific Information

The IK22/5 monoclonal antibody reacts with mouse CD254, also known as TRANCE (TNF-related activation-induced cytokine receptor), RANKL (receptor activator of NF-kappa B ligand), and OPGL (Osteoprotegerin Ligand).
TRANCE is a type II membrane protein (~35 kDa) closely related to TRAIL, FasL, and TNF. It is upregulated by TCR stimulation and expressed abundantly by mouse T cells in thymus and lymph nodes.
TRANCE-deficient mice lack osteoclasts and show defects in T-dendritic and T-B cell interactions, critical for osteoclast differentiation. The TRANCE-RANK interaction regulates expression of the anti-apoptotic molecule Bcl-xL.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Validated on mouse CD254/TRANCE-transfected cells. Recommended ≤0.5 µg/test in 100 µL final volume (10^5–10^8 cells/test). Antibody titration is advised for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD254 (RANKL/TRANCE) is a TNF family cytokine ligand for osteoprotegerin, essential for osteoclast differentiation and activation. It acts as a dendritic cell survival factor and regulates T cell-dependent immune responses.
Activation of T cells induces CD254 expression, promoting osteoclastogenesis and bone loss. It activates anti-apoptotic kinase AKT/PKB via SRC kinase and TRAF6 complex, implicating a role in apoptosis regulation.
Gene disruption in mice causes severe osteopetrosis, lack of osteoclasts, and defects in T/B lymphocyte differentiation and mammary gland development. Two alternatively spliced transcript variants are known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.