Thermo Fisher Scientific TAP Tag Monoclonal Antibody (22F12-1E3), Biotin
TAP 태그 단백질 검출용 Biotin 결합 단클론 항체. Western blot에 최적화되어 있으며 Streptavidin-HRP 검출에 적합. Protein A 정제, 1 mg/mL 농도, PBS 기반 무보존제 용액. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 22F12-1E3 |
| Immunogen | KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536815 |
Available Formats
- Unconjugated (Cat. MA1-108)
- DyLight 488 (Cat. MA1-108-D488)
- DyLight 800 4X PEG (Cat. MA1-108-D800)
- HRP (Cat. MA1-108-HRP)
- Custom conjugation available upon request
Product Specific Information
MA1108-BTIN detects the TEV cleavage peptide sequence.
It has been successfully used for Streptavidin-HRP mediated detection of TAP in Western Blot assays.
Target Information
Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize low-abundance or poorly immunogenic proteins when specific antibodies are unavailable.
Tandem affinity purification (TAP) is an affinity purification method for isolating TAP-tagged proteins together with associated proteins. The TAP tag typically consists of a calmodulin binding peptide (CBP), a tobacco etch virus (TEV) protease cleavage site, and Protein A. This technique is useful for analyzing in vivo protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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