Thermo Fisher Scientific TAP Tag Monoclonal Antibody (22F12-1E3), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

22F12-1E3

Immunogen

KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• DyLight 488
• DyLight 800 4X PEG
• HRP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536815

Product Specific Information

MA1108-BTIN detects the Tev cleavage peptide sequence. MA1108-BTIN has been successfully used for Streptavidin-HRP mediated detection of TAP in Western Blot.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the TAP tag (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.