Thermo Fisher Scientific Laminin beta-2/gamma-1 Monoclonal Antibody (M046)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

-

Immunocytochemistry (ICC/IF)

1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

M046

Immunogen

Clone (M046) was generated from a recombinant human laminin 521 protein that includes a heterotrimer of three subunits laminin alpha5, laminin beta2, and laminin gamma1 subunits.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~1.0 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942703

Product Specific Information

Clone M046 detects native laminin isoforms that include both laminin beta2 and gamma1 subunits. In native immunoblots, the antibody detects laminin 521, laminin 221, and laminin 121, but does not detect lamin 332 and laminin 411. The antibody weakly detects laminin 511. This antibody can be used for immunocytochemical detection of laminin beta2/gamma1 subunits, and can also be used for detection of native laminin isoforms in immunoblots and ELISA.

Target Information

Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.