Thermo Fisher Scientific Rub1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1:1,000-1:5,000

-

Product Specifications

Species Reactivity

Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Rub1 Antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Rub1,uniProtId:Q03919-1,ncbiNodeId:-23,antigenRange:1-77,antigenLength:77,antigenImageFileName:200-401-427_Rub1_Q03919-1_Rabbit.svg,antigenImageFileNamePDP:200-401-427_Rub1_Q03919-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Rub1 Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Target Information

Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg12, and Hub1. Proteins of the second class include parkin, RAD23, and DSK2, and are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDP`s are not proteolytically processed or conjugated to other proteins. Rub1, and the corresponding human homolog Nedd8, are activated by the E1 ubiquitin-activating enzyme UBA2, which forms isopeptide linkages between thioesters. Nedd8 shows 80% homology to ubiquitin. The best known targets of Rub1 modification are members of the cullin family. Cullins are subunits of an E3-ubiquitin ligase complex called the Skp1/Cul1/Cdc53-F-box (SCF). The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein. Rub1 modification may regulate SCF function or localization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.