Thermo Fisher Scientific CD4 Monoclonal Antibody (SK3 (SK-3)), Brilliant Ultra Violet 563, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (SK3 (SK-3)), Brilliant Ultra Violet 563, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.03 µg)/test

Product Specifications

Product Specific Information

Description:
The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by thymocytes, T helper cells, and at low levels on monocytes. CD4 acts as a receptor for HIV. The SK3 clone blocks HIV binding and mixed lymphocyte reactions. SK3 and RPA-T4 antibodies recognize distinct epitopes.

Applications Reported:

• Flow cytometric analysis.

Applications Tested:

• Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells.
• Recommended use: 5 µL (0.03 µg) per test in 100 µL final volume.
• Cell number: 10⁵–10⁸ cells/test.

Fluorochrome Information:
Brilliant Ultra Violet™ 563 (BUV563) is a tandem dye emitting at 564 nm, suitable for cytometers with a 355 nm UV laser. Ensure instrument compatibility.

Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42) or
• Brilliant Stain Buffer™ (Product # 00-4409-75)
  to minimize non-specific interactions.

Light Sensitivity:
Protect from light due to photo-induced oxidation sensitivity.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product # 00-8222-49) or
• 1-step Fix/Lyse Solution (Product # 00-5333-54)
  for up to 3 days at 4°C in the dark with minimal impact on brightness.

Fluorescence Details:

• Excitation: 350 nm
• Emission: 564 nm
• Laser: Ultraviolet Laser

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD4 antigen is involved in MHC class II recognition and HIV co-receptor function. Expressed mainly in T helper lymphocytes, monocytes, macrophages, and dendritic cells.
Detected in thymus, lymph nodes, tonsils, spleen, and certain brain and gut regions.
Functions in T-cell activation via association with TCR complex and Lck kinase.
May mediate neuronal damage in infectious or immune-mediated CNS diseases.
Multiple alternatively spliced transcripts identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

View Additional Formats

• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 605
• eFluor 450
• FITC
• NovaFluor Blue, Red, Violet, Yellow series (multiple wavelengths available)
• PE, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright series (436–780)
• Custom conjugation available upon request

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