Thermo Fisher Scientific VSIG4 Monoclonal Antibody (JAV4), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.06 µg)/test

Product Specifications

Additional Formats

• APC
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

This JAV4 monoclonal antibody recognizes human V-Set and Immunoglobulin domain containing 4 (VSIG4), also known as Complement Receptor of the Immunoglobulin superfamily (CRIg) or Z39Ig.
There are two variants of human VSIG4 resulting from alternative splicing. Isoform 1 is longer and more prevalent than isoform 2, and this JAV4 antibody recognizes both.

VSIG4 is a type I transmembrane glycoprotein structurally related to the B7 family of immune regulatory proteins. It contains one complete V-type Ig domain and one truncated C-type Ig domain.
VSIG4 is exclusively expressed on tissue-resident and tumor-infiltrating macrophages. It binds complement components C3b and iC3b, inhibiting the alternative complement pathway and facilitating phagocytosis of complement-opsonized pathogens.

VSIG4 suppresses T cell activation, proliferation, and IL-2 production, contributing to peripheral T cell tolerance and suppression of inflammation. Expression of VSIG4 on tumor-infiltrating macrophages suggests a role in immune evasion. Pro-inflammatory stimuli such as TNF and LPS down-regulate VSIG4 expression.

The JAV4 antibody recognizes VSIG4 on formaldehyde-fixed and permeabilized cells. It does not cross-react with mouse or rat VSIG4.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on human monocyte-derived macrophages at 5 µL (0.06 µg) per test
  • Defined as the amount of antibody staining a sample in 100 µL volume
  • Cell number range: 10⁵–10⁸ cells/test
• Excitation: 488 nm
• Emission: 519 nm
• Laser: Blue Laser

Target Information

T cell activation by antigen-presenting cells (APCs) is regulated by B7 family members.
VSIG4 acts as a strong negative regulator of murine and human T cell proliferation and IL-2 production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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