Thermo Fisher Scientific Endomucin Monoclonal Antibody (eBioV.7C7 (V.7C7)), Alexa Fluor 488, eBioscience
마우스 Endomucin 인식 단일클론 항체로 Alexa Fluor 488 형광 표지. Flow cytometry, IHC, ICC/IF 등 다양한 응용에 적합. 0.5 mg/mL 액상 형태로 4°C 암소 보관. 연구용으로만 사용 가능.
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카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Endomucin Monoclonal Antibody (eBioV.7C7 (V.7C7)), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 10 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 1.0 µg/test | View 3 publications |
Product Specifications
| Item | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioV.7C7 (V.7C7) |
| Conjugate | Alexa Fluor™ 488 |
| Available Formats | Unconjugated, eFluor 660, PE, Request custom conjugation |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2802331 |
Product Specific Information
Description:
The eBioV.7C7 monoclonal antibody reacts with mouse endomucin, a 75 kDa type I integral membrane protein identified as an endothelial cell marker. Endomucin belongs to the sialomucin family with extensive O-linked glycosylation. It is expressed on endothelial cells except in high endothelial venules (HEV) of secondary lymphoid organs. It is also expressed on CD34⁺c-Kit⁺Sca-1⁺Lin⁻ hematopoietic progenitors capable of multilineage long-term hematopoietic reconstitution.
Applications Reported:
- Reported for use in flow cytometric analysis.
Applications Tested:
- Tested by flow cytometric analysis of bEnd.3 cells.
- Recommended use ≤ 1.0 µg/test (in 100 µL final volume).
- Optimal antibody titration is recommended for best performance.
- Typical cell count range: 10⁵–10⁸ cells/test.
Fluorescence Properties:
- Excitation: 488 nm
- Emission: 519 nm
- Laser: Blue Laser
Target Information
Endomucin (EMCN):
Also known as endothelial sialomucin or mucin-like sialoglycoprotein. It interferes with focal adhesion complex assembly and inhibits cell–extracellular matrix interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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