Thermo Fisher Scientific CD169 (Siglec-1) Monoclonal Antibody (SER-4), Alexa Fluor 488, eBioscience

Applications

Immunohistochemistry (IHC)

• Tested Dilution: Not specified
• Publications: View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 1.0 µg/test
• Publications: View 3 publications

Product Specifications

Additional Formats

• APC-eFluor 780
• eFluor 660
• PE
• Request custom conjugation

Product Specific Information

The SER-4 monoclonal antibody reacts with mouse CD169 (Sialoadhesin, Siglec-1), a 185-kDa member of the Siglec family of adhesion and endocytic receptors that recognize sialylated glycans.
CD169 is expressed on a subset of macrophages in the spleen, liver, lymph node, and bone marrow, as well as on monocytes. It mediates cell-cell adhesion and internalization of sialylated viruses and bacteria, allowing CD169⁺ macrophages to present lipid antigens to iNKT cells and activate them.
It also interacts with regulatory T cells, inhibiting their proliferation. The SER-4 antibody has reported antagonist activity.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells at ≤1.0 µg/test (100 µL final volume). Optimize concentration empirically (10⁵–10⁸ cells/test).
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser

Target Information

Siglecs are membrane-bound lectins (immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Members include sialoadhesin, CD22, and CD33.
Sialoadhesin is a macrophage-specific adhesion molecule expressed on activated macrophages in chronic inflammation and tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지