Thermo Fisher Scientific NR5A1 Monoclonal Antibody (OTI1B4), TrueMAB
Human NR5A1 단백질(아미노산 1-262)을 인식하는 마우스 단클론 항체로, Western blot 및 IHC(P) 검증 완료. 무보존제, 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. 스테로이드 생성 관련 핵심 전사인자 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–262 of human NR5A1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Steroidogenic factor-1 (SF-1) is an orphan nuclear receptor critical for the development and differentiation of endocrine organs such as the hypothalamus, pituitary gland, gonads, and adrenal gland.
SF-1 regulates transcriptional activity of steroidogenic enzymes, particularly those in the cytochrome P450 family.
It contains two activation domains (AF1 and AF2) essential for interactions with nuclear receptors, coactivators, and corepressors, including SRC-1, GRIP1, CREB binding protein (CBP), and SMRT.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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