Thermo Fisher Scientific ERK1/ERK2 Monoclonal Antibody (K.913.4)
ERK1/ERK2 단백질을 검출하는 Rabbit Monoclonal Antibody (Clone K.913.4). Western blot, IHC, ICC, Flow cytometry, IP에 적합. 다양한 종에 반응하며, JNK/SAPK 및 p38 MAP kinase와 교차 반응 없음. 액상 형태로 -20°C 보관.
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 6 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:125–1:500 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100 | View 2 publications |
| Flow Cytometry (Flow) | 1:200–1:800 | – |
| Immunoprecipitation (IP) | 1:50 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, C. elegans, Fruit fly, Hamster, Human, Mink, Mouse, Non-human primate, Pig, Rat, Zebrafish |
| Published Species | Chicken, Human, Pig, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | K.913.4 |
| Immunogen | A synthetic peptide corresponding to residues near the C-terminus of human p44 MAP kinase. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 84 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10982335 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with JNK/SAPK or p38 MAP kinase.
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric G protein–coupled receptors, and transforming agents, activate the ERK1 and ERK2 pathways.
When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase, and activates it as well. Once activated, Raf phosphorylates serine residues in two further kinases, MEK1/2, which in turn phosphorylate tyrosine/threonine residues in extracellular-signal regulated kinase (ERK) 1/2.
Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate transcription factors such as c-Fos and Elk-1.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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