Thermo Fisher Scientific Klotho Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific Klotho Polyclonal Antibody, Biotin

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Klotho. Anti-Human Klotho-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hKlotho by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Klotho (500-P296) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hKlotho.

Western Blot:
To detect hKlotho by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant hKlotho is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package:
500-P296BT-1MG will be provided as 2 × 500 µg.

Target Information

KLOTHO is a systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein abundant in the kidney and brain. In mice, deficiency in KLOTHO expression results in phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy, along with growth retardation, short life-span, and infertility. Transgenic mice overexpressing KLOTHO have extended life spans by inhibiting insulin/IGF1 signaling. KLOTHO regulates calcium/phosphorus homeostasis by inhibiting synthesis of active vitamin D and is identified as a potential tumor suppressor.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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