Thermo Fisher Scientific CD47 Recombinant Rabbit Monoclonal Antibody (001)

Applications

• ELISA (ELISA)
  Tested Dilution: 1:5,000–1:10,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to prevent contamination.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems, developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to larger rabbit immune repertoire

This antibody has specificity for Human CD47.

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein that interacts with several integrins and regulates their functions. Engagement of CD47 by soluble ligands or counter receptors modulates signaling pathways, such as activation of heterotrimeric G proteins.

Functionally, CD47 is involved in increasing intracellular calcium concentration upon cell adhesion to the extracellular matrix. It also serves as a receptor for the C-terminal cell-binding domain of thrombospondin and may play roles in membrane transport and signal transduction.

CD47 exhibits broad tissue distribution but reduced expression on Rh erythrocytes. It acts as a ligand for CD172a (signal regulatory protein alpha, SIRPα), an immune inhibitory receptor on macrophages, preventing phagocytosis of CD47-positive cells. Moreover, the CD47–CD172a system influences cell migration, B cell adhesion, T cell activation, chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.