Thermo Fisher Scientific RAB21 Monoclonal Antibody (OTI5B12), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

RAB21 is a member of the Ras superfamily of small GTP-binding proteins involved in intracellular vesicular trafficking.
In non-polarized Caco-2 cells, RAB21 shows ER-like localization, while in polarized cells it localizes to apical vesicles.
RAB21 associates with integrin subunits and regulates receptor entry via RAB5/RAB21 endosomes.
The GTPase-activating protein p120RasGAP controls cell motility by influencing integrin recycling through competition with RAB21 for overlapping binding sites on the alpha-tail of endocytosed integrin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.