Thermo Fisher Scientific FPR1 (extracellular) Polyclonal Antibody, Atto 488

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:20

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)NFSPWTNDPKERIN, corresponding to amino acid residues 179-192 of human FPR1, 2nd extracellular loop if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FPR1,uniProtId:P21462-1,ncbiNodeId:9606,antigenRange:179-192,antigenLength:350,antigenImageFileName:AFR-001-AG-50UL_FPR1_P21462-1_Rabbit.svg,antigenImageFileNamePDP:AFR-001-AG-50UL_FPR1_P21462-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Atto™ 488 Atto™ 488 Atto™ 488

Excitation/Emission Max

501/523 nm

Form

Lyophilized

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

FPR1 is a member of chemoattractant/chemokine GPCRs expressed mainly on leukocytes, neutrophils and monocytes. FPR1 is a seven transmembrane pertussis toxin-sensitive GPCR and binds to formylated peptides generated by bacteria resulting in leukocyte trafficking to the site of inflammation. Ligand binding to FPR results in activation of multiple effectors including phospholipase C leading to calcium mobilization and PKC activation. Other implications of FPR signaling include polarization of actin cytoskeleton, activation of various integrins, increased cell migration, phagocytosis, degranulation and release of reactive oxygen intermediates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.