
Thermo Fisher Scientific Rabbit anti-Goat IgG (H+L) Secondary Antibody, HRP
Thermo Fisher의 Rabbit anti-Goat IgG (H+L) HRP 결합 2차 항체로, WB, IHC, ELISA에 적합합니다. 고순도 친화 크로마토그래피 정제, 높은 특이성과 감도를 제공하며, 재구성 후 안정적인 장기 보관이 가능합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2534807 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- AP conjugate
- Biotin conjugate
- FITC conjugate
- TRITC conjugate
- Custom conjugation available upon request
Product Specific Information
- Each lot’s sensitivity is confirmed using ELISA.
- Specificity is verified by immunoelectrophoresis (IEP).
- Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Centrifuge to remove particulates.
- Prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
- No reactivity observed to non-immunoglobulin goat serum immunoglobulins.
Target Information
Anti-Goat secondary antibodies are affinity-purified with well-characterized specificity for goat immunoglobulins. They are useful in detection, sorting, or purification of target proteins. Secondary antibodies provide versatility across multiple detection systems (HRP, AP, fluorescence) and enhance sensitivity through signal amplification. Typically, these antibodies are generated by immunizing the host with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, or conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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