Thermo Fisher Scientific Rabbit anti-Goat IgG (H+L) Secondary Antibody, AP
염기성 인산분해효소(AP) 결합 토끼 유래 항-염소 IgG(H+L) 2차 항체. Western blot, IHC, ELISA에 적합하며 높은 특이성과 감도를 제공. 친화 크로마토그래피로 정제되어 신뢰성 높은 검출 가능. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534810 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats
- Biotin
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot is verified by immunoelectrophoresis (IEP).
Target Information
Anti-Goat secondary antibodies are affinity-purified with well-characterized specificity for goat immunoglobulins. They are useful for detection, sorting, or purification of goat antibodies. These secondary antibodies enhance assay versatility, allowing various detection systems (HRP, AP, fluorescence) and increased sensitivity through signal amplification. Typically, they are produced by immunizing the host with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to ensure high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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