Thermo Fisher Scientific Rabbit anti-Chicken IgY (H+L) Secondary Antibody, TRITC
TRITC 결합 Rabbit anti-Chicken IgY (H+L) 2차 항체로, Western blot 및 면역세포염색에 적합. 높은 특이성과 감도를 제공하며, ELISA 및 IEP로 품질 검증됨. 552/578 nm 형광 특성, 장기 보관 시 -20°C 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Chicken IgY (H+L) |
| Conjugate | TRITC |
| Excitation / Emission Max | 552 / 578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C. Avoid freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534806 |
| Target | IgY |
| Antibody Form | Whole Antibody |
Additional Formats
- AP
- Biotin
- FITC
- HRP
- Rhodamine
- Custom conjugation available
Product Specific Information
- Each lot is validated for sensitivity by ELISA and specificity by immunoelectrophoresis (IEP).
- Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Centrifuge to remove particulates. Prepare fresh working dilution daily.
- Product stability: 1 year from date of receipt.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin chicken serum immunoglobulins.
Target Information
Anti-Chicken secondary antibodies are affinity-purified with well-characterized specificity for chicken immunoglobulins.
They are useful for detection, sorting, or purification of target antibodies.
Secondary antibodies provide versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are produced by immunizing the host animal with pooled immunoglobulins from the target species and purified via immunoaffinity chromatography or conjugated to fluorescent labels for enhanced specificity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without authorization.
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