Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), NovaFluor Red 725, eBioscience

Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), NovaFluor Red 725, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 395 / 615 / 737
• Brilliant Violet 650 / 786
• FITC
• Functional Grade
• NovaFluor Blue 510 / 610-70S / 660-120S / 690
• NovaFluor Red 710 / 755
• NovaFluor Yellow 590 / 660 / 755
• PE / PE-Cyanine5 / PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 702
• Request custom conjugation available

Product Specific Information

The W6/32 monoclonal antibody reacts with human MHC class I antigens (HLA-A, B, C). These antigens, associated with beta2-microglobulin, are expressed on all nucleated cells and play a key role in cell-mediated immune response and tumor surveillance.
W6/32 mAb recognizes a non-polymorphic epitope shared among HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11–12 kDa chains. Cross-reactivity is observed in baboon, rhesus, and cynomolgus monkey.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on human peripheral blood cells at 5 µL (0.6 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails prior to combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology: NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures optimized for minimal spillover and spread.

Excitation: 636 nm
Emission: 727 nm
Laser: 633–640 nm (Red)

Target Information

HLA-class I MHC antigens are membrane glycoproteins expressed on nucleated cells, associated with beta2-microglobulin. They present foreign determinants recognized by cytotoxic T cells, essential for antiviral and antitumor defense.
HLA and MHC antibodies are vital in immunopeptidomics, aiding neoantigen identification and characterization via LC-MS/MS analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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