Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Brilliant Violet 711, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 661, 805
• Brilliant Violet 421, 480, 605, 650, 786
• eFluor 450
• FITC
• Functional Grade
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in NK1.1-positive strains (e.g., C57BL, NZB).
Common strains such as BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1. For these strains, use the DX5 monoclonal antibody (Product #14-5971).

Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 shows coexpression of both markers in most cells, with minor DX5⁺PK136⁻ and DX5⁻PK136⁺ populations.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes (≤1.0 µg/test, 100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.
• Fluorochrome: Brilliant Violet™ 711 (BV711), emits at 713 nm, for use with 405 nm violet laser-equipped cytometers.
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to reduce non-specific polymer interactions.
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation Compatibility:
  • Compatible: IC Fixation Buffer (00-8222-49), 1-step Fix/Lyse Solution (00-5333-54)
  • Not compatible: Methanol-based fixation
• Excitation: 407 nm
• Emission: 713 nm
• Laser: Violet Laser

Trademark Notice: BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

NK1.1 (KLRB1, NKR-P1A, CD161) refers to Natural Killer (NK) cells, which mediate cytotoxicity and cytokine secretion upon immune stimulation.
The KLRB1 protein is a type II membrane protein with a C-type lectin extracellular domain, transmembrane region, and cytoplasmic tail.
Its receptor, NKR-P1A (encoded by KLRB1), recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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