Thermo Fisher Scientific SUMO1 Monoclonal Antibody (21C7)
SUMO1 단백질 및 SUMO화 관련 단백질 검출용 단클론 항체. Human, Mouse, Rat 반응성. Western blot, IHC, ICC, ELISA 등 다양한 응용 가능. Unconjugated 형태, Protein A 정제, 0.5 mg/mL 농도.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | 45 |
| Immunohistochemistry (IHC) | Assay-dependent | 8 |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | 1 |
| Immunocytochemistry (ICC/IF) | – | 21 |
| ELISA | 0.1–1.0 µg/mL | 1 |
| Immunoprecipitation (IP) | – | 10 |
| in situ PLA (PLA) | – | 1 |
| Gel Shift (GS) | – | 1 |
| Miscellaneous PubMed (Misc) | – | 17 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Hamster, Human, Mink, Mouse, Non-human primate, Rat, Rhesus monkey, Xenopus |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 21C7 |
| Immunogen | Full length recombinant GMP-1 (SUMO1, UniProt ID: P63165-1, antigen range: 1–101, antigen length: 101) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533109 |
Product Specific Information
This monoclonal antibody specifically detects the unconjugated (~17 kDa) form of SUMO-1/GMP-1 and proteins covalently ligated to GMP-1 (e.g., RanGAP-1).
Lysates tested: Rat liver nuclear envelopes, total lysates from NIH 3T3 and HeLa cells.
Target Information
SUMO1 is an ubiquitin-like protein that can be covalently attached to proteins as a monomer or lysine-linked polymer.
Attachment requires activation by the E1 complex (SAE1-SAE2), linkage to E2 enzyme (UBE2I), and can be promoted by E3 ligases (PIAS1–4, RANBP2, CBX4).
SUMO1 modification regulates nuclear transport, DNA replication and repair, mitosis, and signal transduction.
It targets RANGAP1 to the nuclear pore complex protein RANBP2, and polymeric SUMO1 chains can be polyubiquitinated for proteasomal degradation.
SUMO1 may also regulate gene networks involved in palate development, and mutations can cause non-syndromic orofacial cleft 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 33-2400_SUMO1_P63165-1_House_mouse.svg, 33-2400_SUMO1_P63165-1_House_mouse_PDP.jpeg)
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