Thermo Fisher Scientific SUMO1 Monoclonal Antibody (21C7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 45 publications 45 publications

Immunohistochemistry (IHC)

Assay-dependent

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 21 publications 21 publications

ELISA (ELISA)

0.1-1.0 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 10 publications 10 publications

in situ PLA (PLA)

-

View 1 publication 1 publication

Gel Shift (GS)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Hamster, Human, Mink, Mouse, Non-human primate, Rat, Rhesus monkey, Xenopus

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

21C7

Immunogen

Full length recombinant GMP-1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SUMO1,uniProtId:P63165-1,ncbiNodeId:9606,antigenRange:1-101,antigenLength:101,antigenImageFileName:33-2400_SUMO1_P63165-1_House_mouse.svg,antigenImageFileNamePDP:33-2400_SUMO1_P63165-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533109

Product Specific Information

This monoclonal antibody can be used to specifically detect the unconjugated (~17 kDa) form of SUMO-1/GMP-1, as well as proteins covalently ligated to GMP-1 (e.g., RanGAP-1). Lysates tested: Rat liver nuclear envelopes, total lysates derived from NIH 3T3 and HeLa cells.

Target Information

SUMO1 is an ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment, via an isopeptide bond, to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO1 is involved, for instance, in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. SUMO1 may also regulate a network of genes involved in palate development. Mutations in the gene can result in non-syndromic orofacial cleft 10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.