Thermo Fisher Scientific p27 Kip1 Monoclonal Antibody (p27-11D11)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

0.1-1.0 µg/mL

-

Immunoprecipitation (IP)

5 µg

-

Product Specifications

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

p27-11D11

Immunogen

Full-length, human recombinant p27 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

Target Information

p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.