Thermo Fisher Scientific CD40 Monoclonal Antibody (OTI6F6)

Applications

Flow Cytometry (Flow)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The CD40 antigen is a single-chain glycoprotein belonging to the tumor necrosis factor/nerve growth factor superfamily. It shows homology to the Hodgkin’s disease-associated antigen CD30. CD40 is expressed on all B cells except plasma cells and is also found on epithelial cells, carcinomas, and lymphoid dendritic cells.
CD40 plays a crucial role in immune and inflammatory responses, including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
AT-hook transcription factor AKNA regulates CD40 expression, mediating homotypic cell interactions. TNFR2 adaptor protein interacts with CD40 to mediate signal transduction.
The CD40-CD40L interaction is required for amyloid-beta-induced microglial activation, implicated in early Alzheimer’s disease pathogenesis.
Two alternatively spliced transcript variants encoding distinct isoforms have been reported.
Diseases associated with CD40 dysfunction include Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.