Thermo Fisher Scientific SCK Polyclonal Antibody
SCK 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western Blot 및 Immunocytochemistry에 적합. Human, Mouse, Rat에 반응하며 항원 친화 크로마토그래피로 정제됨. 액상 형태, 1 mg/mL 농도. VEGF 신호전달 관련 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 339–384 of human SCK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2553883 |
Product Specific Information
- Detects endogenous SCK protein at approximately 68 kDa.
- Purity >95% by SDS-PAGE.
Target Information
Src homology 2 (SH2) domains bind specifically to tyrosine-phosphorylated proteins involved in signal transduction.
Shc-like protein (Sck) is a neuronal adaptor protein containing an N-terminal PTB (phosphotyrosine binding) domain, a collagen homology (CH) domain, and a conserved C-terminal SH2 domain.
Human Sck transcripts are abundant in liver, pancreas, prostate, and ovary. In vascular endothelial cells, Sck participates in VEGF-induced signal transduction. Treatment of HUVEC cells with VEGF induces recruitment of Sck to tyrosine-1175 of the kinase insert domain-containing receptor (KDR) and enhances Sck tyrosine phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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