Thermo Fisher Scientific GST Monoclonal Antibody (2F10B9), THE
GST 단백질 검출용 Mouse monoclonal antibody로, Western blot, ELISA, Immunoprecipitation에 적합합니다. 단백질 발현 및 정제 과정에서 GST-tagged 단백질 확인에 유용하며, 단일 단계로 높은 특이성의 결과를 제공합니다.
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- A00865xxx (3개 옵션)
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카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific GST Monoclonal Antibody (2F10B9), THE
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–1 µg/mL | – |
| ELISA | 0.05–0.2 µg/mL | – |
| Immunoprecipitation (IP) | 1 µg/mL | – |
| Miscellaneous (PubMed) | – | View 19 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2F10B9 |
| Immunogen | Recombinant GST protein (26 kD) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C or –80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats:
Product Specific Information
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles.
GST serves as an ideal fusion partner for production of foreign proteins in expression systems due to its high expression levels in E. coli.
Fusion proteins can be purified in a single step because the GST portion binds tightly to immobilized glutathione, and elution is achieved using free glutathione.
The GST tag can be removed from the protein of interest using site-specific proteases.
Antibodies against GST are useful for detecting fusion proteins during purification and for confirming cleavage of GST from the target protein.
Target Information
Glutathione S-transferase (GST) is a 26 kDa enzyme found in both eukaryotes and prokaryotes, catalyzing various reactions.
The GST gene fusion system enables purification of GST-fusion proteins via their strong affinity for glutathione, facilitating studies of protein–protein interactions.
This system is widely used in numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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