Thermo Fisher Scientific Phospho-CREB (Thr100) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Antibody detects endogenous levels of CREB only when phosphorylated at Thr100.

Target Information

CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to various extracellular signals such as neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB depends on phosphorylation at serine 133, mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase, and MSK1. Although CREB binds DNA regardless of its phosphorylation state, only the phosphorylated form functions as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator, interacts with CREB at serine 133. CREB is involved in cellular processes including synchronization of circadian rhythmicity, adipocyte differentiation, and learning and memory. In humans, the CREB gene is located on chromosome 2q. CREB dysfunction is associated with diseases such as Alzheimer’s Disease, histiocytoma, and soft tissue melanoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.